Chemical exchange between dentin pre-tratment with CPP-ACP and GIC on demineralized dentin in deciduous molars

Gisele Fernandes Dias, Fabiana Bucholdz Teixeira Alves, Gabriela Silveira, Fábio André dos Santos

Resumo


Aim: To assess the effect of the chemical exchange between restorations using high-viscosity glass ionomer cement (GIC) and the casein phosphopeptide amorphous calcium phosphate CPP-ACP treatment on primary demineralized dentin by analyzing the hardness changes caused by calcium, phosphate and fluoride uptake. Methods: 40 deciduous molars were selected and randomly assigned to four groups according to dentin pretreatment and dentin condition. Class I cavity preparations were performed in 40 sound primary molar samples, equally divided into groups G1 (sound dentin) and G2 (demineralized dentin). Sub-groups (n=10) were set in order to aid in investigating the isolated GIC action or its association with CPP-ACP. This study was conducted in vitro and assessed the chemical exchange under two conditions, namely: sound and demineralized dentin (pH cycling); This in vitro study examined the mechanical and chemical exchange under two conditions – sound and demineralized dentin (pH cycling) – to simulate the mineral loss that occurs for the caries lesion. The 40 teeth first received a topical application of ACP-CPP and a restoration of high viscosity GIC. The 20 teeth assigned to the groups (G1 and G2) were only restored with GIC. The specimens were sliced and prepared for Knoop hardness test (KHN), Micro Raman, and FEG microhardness analysis groups. The statistical analysis used ANOVA and Bonferroni post-test at a 5% significance level. EDS (Dispersive Energy Spectroscopy) and FEG (High-resolution scanning electron microscope) data were qualitatively described. Results: Increased hardness was observed in all sites that had direct contact with GIC in the sound and demineralized dentin samples in all groups (p<0.001); microhardness showed no differences after CPP-ACP application (p>0.05). The direct contact between GIC in sound and demineralized dentins resulted in an increased phosphate peak in the FEG and EDS evaluations. Conclusion: ACP-CPP associated with GIC showed no increase in microhardness values of the demineralized dentin substrate. The exchange between the GIC and the demineralized dentin may induce changes in the mechanical properties of the substrate and in the uptake of mineral ions.

Uniterms: dentin; prevention; caries; remineralization; demineralization; biomechanics

Referências


(01) American Academy of Pediatric Dentistry. Reference manual: guidelines on pulp therapy for primary and immature permanent teeth. Pediatr Dent. 2010; 31:179-86.

(02) Pashley DH, Pashley EL, Carvalho RM, Tay FR. The effects of dentin permeability on restorative dentistry. Dent Clin North Am. 2002;46(2):211-45.

(03) Ngo HC, Mount G, McIntyre J, Do L. An in vitro model for the study of chemical exchange between glass ionomer restorations and partially demineralized dentin using a minimally invasive restorative technique. J Dent. 2011 Dec;39 Suppl 2:S20-6.

(04) Zhang Q, Zou J, Yang R, Zhaou, X. Remineralization effects of casein phosphopeptide amouphous calcium phosphate crème on artificial early enamel lesions of primary teeth. Intern J of Paed Dent. 2011;21:374-81.

(05) Llena C, Forner L, Baca P. Anticariogenicity of casein phosphopeptide-amorphous calcium phosphate: a review of the literature. J Contemp Dent Pract. 2009;3(10):1-9.

(06) Neeser JR, Golliard M, Woltz A, Rouvet M, Dillmann ML, Guggenheim B. In vitro modulation of oral bacterial adhesion to saliva coated hydroxyapatite beads by milk casein derivatives. Oral Microbiol Immunol. 1994 Aug;9(4):193-201.

(07) Marquezan M, Correa FNP, Sanabe ME, Rodrigues Filho LE, Hebling J, Guedes-Pinto AC, et al. Artificial methods of dentine caries induction: a hardness and morphological comparative study. Arch Oral Biol. 2009;54(12):1111-7.

(08) Galo R, Contente MM, Bosatto MC. Wear of two pit and fissure sealants in contact with primary teetn. Eur J Dent. 2014;8:241-8.

(09) Sauro S, Thompson I, Watson TF. Effects of common dental materials used in preventive or operative dentistry on dentin permeability and remineralization. Oper Dent. 2011;36(2):222-30.

(10) Kumar VLN, Ittagarun A, King NM. The effect of casin phosphopeptide-amourphous calcium phosphate on remineralization of articial caries-like lseions: an in vitro study. Aust Dent J. 2008;53(1):34-40.

(11) Bezerra AC, Novaes RC, Faber J, Frencken JE, Leal SC. Ion concentration adjacent to glass-ionomer restorations in primary molars. Dent Mater. 2012; 28(11):259-63.

(12) Zhang S1, Chen T, Ge LH. Scanning electron microscopy study of cavity preparation in deciduous teeth using the Er: YAG laser with different powers. Lasers Med Sci. 2012 Jan;27(1):141-4.

(13) Malkoc MA, Sevimay M. Evaluation onf mineral content of dentin treated with desensitizing agents and neodymium yttrium-aluminium-garnet (Nd: YAG) laser. Laser in Med Sci. 2012;23(4):743-8.

(14) Todd MA, Staley RN, Kanellis MJ, Donly KJ, Wefel JS. Effect of a fluoride varnish on demineralization adjacent to orthodontic brackets. Am J Orthod Dentof Orthop. 1999;116(2):159-67.

(15) Ten Cate JM, Duijster PP. Alternating demineralization and remineralization of artificial enamel lesions. Caries Res. 1982;16:201-10.

(16) Diamanti I, Koletsi-kounari H, Mamai-homata E, Vougiouklakis G. In vitro evaluation of fluoride and calcium sodium phosphosilicate toothpastes on root dentine caries lesions. J Dent. 2011;39(9):619-28.

(17) Vitorino R, Lobo MJ, Duarte JR, Ferrer-Correia AJ, Domingues PM, Amado F M. The role of salivary peptides in dental caries. Biomed Chromatogr. 2005;19(3):214-22.

(18) Oshiro M, Yamaguchi K, Takamizawa T, Inage H, Watanabe T, Irokawa A, et al. Effect of CPP-ACP paste on tooth mineralization: an FE-SEM study. J of Oral Sci. 2007;49(2):115-20.

(19) Pulido MT, Wefel JS, Hernandez MM, Denehy GE, Guzman-Armstrong S, Chalmers JM, et al. The inhibitory effect of MI paste, fluoride and a combination of both on the progression of artificial caries-like lesions in enamel. Oper Dent. 2008;33:550-5.

(20) Yamaguchi K, Miyazaki M, Takamizawa T, Inage, H, Kurokawa H. Ultrasonic determination of the effect of casein phosphopeptide-amorphous calcium phosphate paste of the demineralization of bovine dentin. Caries Res. 2007;41(3):204-7.

(21) Rahiotics C, Vougiouklakis G. Effect of a CPP-ACP agent on the demineralization and remineralization of dentine in vitro. J Dent. 2007;35(8): 695-98.

(22) Ferrazzano GF, Amato I, Cantile T, Sangianantoni G, Ingenito A. In vivo remineralising effect of GC Tooth mousse on eraly dental enamil lesions: SEM analysis. Int Int Dent J. 2011 Aug;61(4):210-6.

(23) Langhorst SE, O’Donnell JNR, Skrtic D. In vitro remineralization of enamel by polymeric amorphous calciu phosphate composite: quantitative microradiographic study. Dent Mater. 2009;25:884-91.

(24) Erdem AP, Sepet E, Avshalom T, Gutkin V, Steinberg D. Effect of CPP-ACP and APF on Streptococcus mutans biofilm: A laboratory study. Am J Dent. 2011 Apr;24(2):119-23.

(25) Ranjitkar S, Rodriguez JM, Kaidonis JA, Richards LC, Townsend GC, Bartlett DW. The effect of casein phohphopeptide-amouphous calcium phosphate on erosive enamel and dentine wear by toothbrush abrasion. J Dent. 2009 Apr;37(4):250-4.


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